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Anti -6 X His Tag Rabbit pAb

货号:GB111251

品牌:Servicebio

价格:¥650

规格: 100μl

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Product Information

 

Description

6 X His Tag rabbit polyclonal

Synonyms

6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED

Immunogen

KLH conjugated Synthetic peptide corresponding to HHHHHH (6* His)

Isotype IgG
Purity Affinity purification

 


 

Applications

 

Applications

WB

Dilution

1: 500-1: 1000

Positive tissue

His-Tag fusion protein

 


 

Background

 

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.


 

Images

 

Western blot analysis of 6*His Tag (GB111251) at dilution of 1: 1000
Lane 1, 2, 3, 6, 7: The different His-Tag fusion proteins
Lane 4, 5: The different GST-Tag fusion proteins

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.