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Anti -SAE1 Rabbit pAb

货号:GB111191

品牌:Servicebio

价格:¥500

规格: 100 μl

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Product Information

 

Description

SAE1 rabbit polyclonal

Protein full name
SUMO-activating enzyme subunit 1

Synonyms

Ubiquitin-like 1-activating enzyme E1A, Sae1, SUMO-activating enzyme subunit 1, Uble1a, Aos1, Sua1, N-terminally processed, Ubl1a1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse SAE1

Isotype IgG
Purity Affinity purification
Subcellular location
 
Nucleus
 

Predicted MW.

39 kDa

Observed MW.

39 kDa

Uniprot ID

Q9UBE0, Q9R1T2, Q6AXQ0

 


 

Applications

 

Applications

WB

IHC
 
IF
 
Species

Human, Mouse, Rat

Human, Mouse, Rat Human, Mouse, Rat
Dilution

1: 500-1: 1000

1: 1500-1: 3000 1: 750-1: 1500
Positive tissue

testis

colon, breast, brain, small intestine, testis breast, lung, brain, immune SD rat brain, testis

 


 

Background

 

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
 


 

Images

 

Western blot analysis of SAE1 (GB111191) at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: MCF7 cell lysate
Lane 3: A549 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC12 cell lysate
Lane 6: HEK293 cell lysate
Lane 7: Mouse testis tissue lysate
Lane 8: Rat testis tissue lysate
 

   

Immunohistochemistry analysis of paraffin-embedded human colon using SAE1 (GB111191) at dilution of 1: 2400

 
   

Immunohistochemistry analysis of paraffin-embedded rat colon using SAE1 (GB111191) at dilution of 1: 2400

 
   

Immunohistochemistry analysis of paraffin-embedded human breast using SAE1 (GB111191) at dilution of 1: 2400

 
   

Immunohistochemistry analysis of paraffin-embedded mouse brain using SAE1 (GB111191) at dilution of 1: 2400

 
   

Immunohistochemistry analysis of paraffin-embedded rat brain using SAE1 (GB111191) at dilution of 1: 2400
 
   

Immunohistochemistry analysis of paraffin-embedded mouse small intestine using SAE1 (GB111191) at dilution of 1: 2400

 
   

Immunohistochemistry analysis of paraffin-embedded mouse testis using SAE1 (GB111191) at dilution of 1: 2400

 
   

Immunofluorescent analysis of paraffin-embedded human breast using SAE1 (GB111191) at dilution of 1: 1100
 
   

Immunofluorescent analysis of paraffin-embedded mouse lung using SAE1 (GB111191) at dilution of 1: 1100

 
   

Immunofluorescent analysis of paraffin-embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1100

 
   

Immunofluorescent analysis of paraffin-embedded immune rat brain using SAE1 (GB111191) at dilution of 1: 1100
 
   

Immunofluorescent analysis of paraffin-embedded rat testis using SAE1 (GB111191) at dilution of 1: 1100
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.