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Anti -Chk2 Mouse mAb

货号:GB12575

品牌:Servicebio

价格:¥650

规格: 100μl

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Product Information

 

Description

Chk2 mouse monoclonal

Protein full name

Serine/ threonine-protein kinase Chk2

Synonyms

CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, checkpoint kinase 2

Immunogen

Recombinant protein corresponding to Mouse Chk2 

Isotype IgG
Purity Affinity purification
Subcellular location

Nucleus

Uniprot ID

O96017

 


 

Applications

 

Applications

IHC

IF

Species

Human

Human

Dilution

1: 300-1: 600

1: 150-1: 300

Positive tissue

colon, colon cancer,stomach cancer

colon, colon cancer,stomach cancer

 


 

Background

 

CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2. Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs). Thus, CHK2’ s inhibition of the CDC25 phosphatases prevents entry of the cell into mitosis.

 

 


 

Images

 

Immunohistochemistry analysis of paraffin-embedded human colon using
Chk2 (GB12575) at dilution of 1: 1000

   

Immunohistochemistry analysis of paraffin-embedded human colon cancer
using Chk2 (GB12575) at dilution of 1: 1000

   

Immunohistochemistry analysis of paraffin-embedded human stomach cancer
using Chk2 (GB12575) at dilution of 1: 1000

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.