赛维尔生物服务、产品资料库

赛维尔生物

TEL:400-6027-178

赛维尔生物

一抗

  1. 首页
  2. 全部分类
  3. 产品
  4. 抗体
  5. 一抗
  6. 内容

Anti -NEDD4 Rabbit pAb

货号:GB111057

品牌:Servicebio

价格:¥650

规格: 100μl

下载说明书
附件下载地址: ×
/e/DownSys/DownSoft/?classid=18&id=2738&pathid=0

Product Information

 

Description

NEDD4 rabbit polyclonal

Protein full name

E3 ubiquitin-protein ligase NEDD4

Synonyms

HECT-type E3 ubiquitin transferase NEDD4, Kiaa0093, Neural precursor cell expressed developmentally down-regulated protein 4,Nedd4, Nedd4-1, Nedd4a

Immunogen

Recombinant protein corresponding to Mouse NEDD4

Isotype IgG
Purity Affinity purification

Predicted MW.

103 kDa

Observed MW.

120 kDa

Uniprot ID

P46935, Q62940

 


 

Applications

 

Applications

WB

Species

Mouse, Rat

Dilution

1: 500-1: 1000

Positive tissue

brain, lung,cerebral cortex

 


 

Background

 

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/kDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

 


 

Images

 

Western blot analysis of NEDD4 (GB111057) at dilution of 1: 1000

Lane 1: NIH/3T3 cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse cerebral cortex tissue lysate
Lane 4: Mouse lung tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: Rat cerebral cortex tissue lysate

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.